Label-free optical control of arterial contraction

Myunghwan Choi; Jonghee Yoon; Chulhee Choi

doi:10.1117/12.841505

11 February 2010 Label-free optical control of arterial contraction

Myunghwan Choi, Jonghee Yoon, Chulhee Choi

Proceedings Volume 7562, Optical Interactions with Tissues and Cells XXI; 75620G (2010) https://doi.org/10.1117/12.841505
Event: SPIE BiOS, 2010, San Francisco, California, United States

Abstract

Blood vessels, especially in the brain, dynamically change the diameters over time to provide sufficient blood supply where needed. At present, there is no technique that allows noninvasive control of vascular diameter in vivo. Here we report that label-free irradiation of femtosecond pulsed laser can trigger blood vessel contraction in vivo. In response to laser irradiation, cultured vascular smooth muscle cell showed a rapid increase in calcium concentration followed by the cell contraction. In a murine thinned skull window model, laser irradiation focused in the arterial vessel wall caused localized vascular contraction followed by recovery. Nonlinear nature of the pulsed laser allowed highly specific targeting of subcortical vessels without affecting the surrounding region. We propose that femtosecond pulsed laser irradiation will be a useful experimental tool in the field of vascular biology.

Citation Download Citation

Myunghwan Choi, Jonghee Yoon, and Chulhee Choi "Label-free optical control of arterial contraction", Proc. SPIE 7562, Optical Interactions with Tissues and Cells XXI, 75620G (11 February 2010); https://doi.org/10.1117/12.841505

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