Investigation of pore structure and cell distribution in EH-PEG hydrogel scaffold using optical coherence tomography and fluorescence microscopy

Chao-Wei Chen; Martha W. Betz; John P. Fisher; Andrew Paek; James Jiang; Hongzhou Ma; Alex Cable; Yu Chen

doi:10.1117/12.840809

11 February 2010 Investigation of pore structure and cell distribution in EH-PEG hydrogel scaffold using optical coherence tomography and fluorescence microscopy

Chao-Wei Chen, Martha W. Betz, John P. Fisher, Andrew Paek, James Jiang, Hongzhou Ma, Alex Cable, Yu Chen

Author Affiliations +

Proceedings Volume 7566, Optics in Tissue Engineering and Regenerative Medicine IV; 756603 (2010) https://doi.org/10.1117/12.840809
Event: SPIE BiOS, 2010, San Francisco, California, United States

Abstract

Macroporous EH-PEG hydrogels fabricated by porogen-leaching method are characterized by optical coherence tomography (OCT). High-resolution OCT visualizes the microstructures of the engineered tissue scaffolds in threedimensions. It also enables subsequent image processing to investigate several key morphological design parameters for macroporous scaffolds. Image processing algorithms are then presented to automatically quantify the pore size, porosity, and pore interconnectivity. The results indicated that those parameters highly depend on the porogen size. Further, fluorescence imaging was conducted to monitor the population of labeled human mesenchymal stem cells (hMSCs) loaded on the surface of the scaffolds. The results revealed the hMSCs' viability as well as their infiltration into the scaffold. The effect of infiltration is more profound in the scaffold of larger pore sizes, in accordance with the result suggested by image analysis.

Citation Download Citation

Chao-Wei Chen, Martha W. Betz, John P. Fisher, Andrew Paek, James Jiang, Hongzhou Ma, Alex Cable, and Yu Chen "Investigation of pore structure and cell distribution in EH-PEG hydrogel scaffold using optical coherence tomography and fluorescence microscopy", Proc. SPIE 7566, Optics in Tissue Engineering and Regenerative Medicine IV, 756603 (11 February 2010); https://doi.org/10.1117/12.840809

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