Frequency modulation coherent anti-Stokes Raman scattering (FM-CARS) microscopy based on spectral focusing of chirped laser pulses

Bi-Chang Chen; Jiha Sung; Sang-Hyun Lim

doi:10.1117/12.841497

26 February 2010 Frequency modulation coherent anti-Stokes Raman scattering (FM-CARS) microscopy based on spectral focusing of chirped laser pulses

Bi-Chang Chen, Jiha Sung, Sang-Hyun Lim

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Proceedings Volume 7569, Multiphoton Microscopy in the Biomedical Sciences X; 756909 (2010) https://doi.org/10.1117/12.841497
Event: SPIE BiOS, 2010, San Francisco, California, United States

Abstract

We demonstrate a new CARS microscopy method based on fast switching of effective vibrational excitation frequency from chirped femtosecond laser pulses. Broadband pump and Stokes pulses excite a single vibrational mode with a high spectral resolution when the two pulses are identically chirped and their pulse durations are approaching the dephasing time of the excited vibrational state. This "spectral focusing" mechanism is applied to CARS microscopy with a single broadband Ti:Sapphire laser. The vibrational excitation frequency is controlled simply by the time delay between the pump and Stokes pulses and fast switching of the excitation frequency (~100 kHz) is achieved with a Pockels cell and polarization optics. Lock-in detection of the difference between the two CARS signals at nearby vibrational frequencies not only eliminates the non-resonant background but also generates a spectral line shape similar to the spontaneous Raman scattering. We demonstrate both micro-spectroscopy and vibrational imaging with various samples.

Citation Download Citation

Bi-Chang Chen, Jiha Sung, and Sang-Hyun Lim "Frequency modulation coherent anti-Stokes Raman scattering (FM-CARS) microscopy based on spectral focusing of chirped laser pulses", Proc. SPIE 7569, Multiphoton Microscopy in the Biomedical Sciences X, 756909 (26 February 2010); https://doi.org/10.1117/12.841497

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KEYWORDS

Microscopy

Frequency modulation

Pulsed laser operation

Fast packet switching

Imaging spectroscopy

Laser applications

Polarization

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