Snapshot spectral imaging system

Thomas Arnold; Martin De Biasio; Gerald McGunnigle; Raimund Leitner

doi:10.1117/12.843193

24 February 2010 Snapshot spectral imaging system

Thomas Arnold, Martin De Biasio, Gerald McGunnigle, Raimund Leitner

Proceedings Volume 7570, Three-Dimensional and Multidimensional Microscopy: Image Acquisition and Processing XVII; 75700K (2010) https://doi.org/10.1117/12.843193
Event: SPIE BiOS, 2010, San Francisco, California, United States

Abstract

Spectral imaging is the combination of spectroscopy and imaging. These fields are well developed and are used intensively in many application fields including industry and the life sciences. The classical approach to acquire hyper-spectral data is to sequentially scan a sample in space or wavelength. These acquisition methods are time consuming because only two spatial dimensions, or one spatial and the spectral dimension, can be acquired simultaneously. With a computed tomography imaging spectrometer (CTIS) it is possible to acquire two spatial dimensions and a spectral dimension during a single integration time, without scanning either spatial or spectral dimensions. This makes it possible to acquire dynamic image scenes without spatial registration of the hyperspectral data. This is advantageous compared to tunable filter based systems which need sophisticated image registration techniques. While tunable filters provide full spatial and spectral resolution, for CTIS systems there is always a tradeoff between spatial and spectral resolution as the spatial and spectral information corresponding to an image cube is squeezed onto a 2D image. The presented CTIS system uses a spectral-dispersion element to project the spectral and spatial image information onto a 2D CCD camera array. The system presented in this paper is designed for a microscopy application for the analysis of fixed specimens in pathology and cytogenetics, cell imaging and material analysis. However, the CTIS approach is not limited to microscopy applications, thus it would be possible to implement it in a hand-held device for e.g. real-time, intra-surgery tissue classification.

Citation Download Citation

Thomas Arnold, Martin De Biasio, Gerald McGunnigle, and Raimund Leitner "Snapshot spectral imaging system", Proc. SPIE 7570, Three-Dimensional and Multidimensional Microscopy: Image Acquisition and Processing XVII, 75700K (24 February 2010); https://doi.org/10.1117/12.843193

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KEYWORDS

Imaging spectroscopy

Imaging systems

Data acquisition

Computed tomography

Spectroscopy

Reconstruction algorithms

Microscopes

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