Investigating the spectral characteristics of backscattering from heterogeneous spheroidal nuclei using broadband finite-difference time-domain simulations

Guo-Shan Chao; Kung-Bin Sung

doi:10.1117/12.841876

26 February 2010 Investigating the spectral characteristics of backscattering from heterogeneous spheroidal nuclei using broadband finite-difference time-domain simulations

Guo-Shan Chao, Kung-Bin Sung

Author Affiliations +

Proceedings Volume 7573, Biomedical Applications of Light Scattering IV; 75730E (2010) https://doi.org/10.1117/12.841876
Event: SPIE BiOS, 2010, San Francisco, California, United States

Abstract

Backscattered light spectra have been used to extract size distribution of cell nuclei in epithelial tissues for noninvasive detection of precancerous lesions. In existing experimental studies, size estimation is achieved by assuming nuclei as homogeneous spheres or spheroids and fitting the measured data with models based on Mie theory. However, the validity of simplifying nuclei as homogeneous spheres has not been thoroughly examined. In this study, we investigate the spectral characteristics of backscattering from models of spheroidal nuclei under plane wave illumination using three-dimensional finite-difference time-domain (FDTD) simulation. A modulated Gaussian pulse is used to obtain wavelength dependent scattering intensity with a single FDTD run. The simulated model of nuclei consists of a nucleolus and randomly distributed chromatin condensation in homogeneous cytoplasm and nucleoplasm. The results show that backscattering spectra from spheroidal nuclei have similar oscillating patterns to those from homogeneous spheres with the diameter equal to the projective length of the spheroidal nucleus along the propagation direction. The strength of backscattering is enhanced in heterogeneous spheroids as compared to homogeneous spheroids. The degree of which backscattering spectra of heterogeneous nuclei deviate from Mie theory is highly dependent on the distribution of chromatin/nucleolus but not sensitive to nucleolar size, refractive index fluctuation or chromatin density.

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Guo-Shan Chao and Kung-Bin Sung "Investigating the spectral characteristics of backscattering from heterogeneous spheroidal nuclei using broadband finite-difference time-domain simulations", Proc. SPIE 7573, Biomedical Applications of Light Scattering IV, 75730E (26 February 2010); https://doi.org/10.1117/12.841876

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KEYWORDS

Backscatter

Finite-difference time-domain method

Light scattering

Optical spheres

Mie scattering

Refractive index

3D modeling

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