Correlation between light scattering signal and tissue reversibility in rat brain exposed to hypoxia

Satoko Kawauchi; Shunichi Sato; Yoichi Uozumi; Hiroshi Nawashiro; Miya Ishihara; Makoto Kikuchi

doi:10.1117/12.841489

26 February 2010 Correlation between light scattering signal and tissue reversibility in rat brain exposed to hypoxia

Satoko Kawauchi, Shunichi Sato, Yoichi Uozumi, Hiroshi Nawashiro, Miya Ishihara, Makoto Kikuchi

Proceedings Volume 7573, Biomedical Applications of Light Scattering IV; 75730U (2010) https://doi.org/10.1117/12.841489
Event: SPIE BiOS, 2010, San Francisco, California, United States

Abstract

Light scattering signal is a potential indicator of tissue viability in brain because cellular and subcellular structural integrity should be associated with cell viability in brain tissue. We previously performed multiwavelength diffuse reflectance measurement for a rat global ischemic brain model and observed a unique triphasic change in light scattering at a certain time after oxygen and glucose deprivation. This triphasic scattering change (TSC) was shown to precede cerebral ATP exhaustion, suggesting that loss of brain tissue viability can be predicted by detecting scattering signal. In the present study, we examined correlation between light scattering signal and tissue reversibility in rat brain in vivo. We performed transcranial diffuse reflectance measurement for rat brain; under spontaneous respiration, hypoxia was induced for the rat by nitrogen gas inhalation and reoxygenation was started at various time points. We observed a TSC, which started at 140 ± 15 s after starting nitrogen gas inhalation (mean ± SD, n=8). When reoxygenation was started before the TSC, all rats survived (n=7), while no rats survived when reoxygenation was started after the TSC (n=8). When reoxygenation was started during the TSC, rats survived probabilistically (n=31). Disability of motor function was not observed for the survived rats. These results indicate that TSC can be used as an indicator of loss of tissue reversibility in brains, providing useful information on the critical time zone for treatment to rescue the brain.

Citation Download Citation

Satoko Kawauchi, Shunichi Sato, Yoichi Uozumi, Hiroshi Nawashiro, Miya Ishihara, and Makoto Kikuchi "Correlation between light scattering signal and tissue reversibility in rat brain exposed to hypoxia", Proc. SPIE 7573, Biomedical Applications of Light Scattering IV, 75730U (26 February 2010); https://doi.org/10.1117/12.841489

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KEYWORDS

Brain

Light scattering

Diffuse reflectance spectroscopy

Tissue optics

Oxygen

Scattering

Hypoxia

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