Smart oxygen cuvette for optical monitoring of dissolved oxygen in biological blood samples

Harish Dabhi; Suresh Kumar Alla; Mahmoud R. Shahriari

doi:10.1117/12.840928

16 February 2010 Smart oxygen cuvette for optical monitoring of dissolved oxygen in biological blood samples

Harish Dabhi, Suresh Kumar Alla, Mahmoud R. Shahriari

Proceedings Volume 7576, Reporters, Markers, Dyes, Nanoparticles, and Molecular Probes for Biomedical Applications II; 75761B (2010) https://doi.org/10.1117/12.840928
Event: SPIE BiOS, 2010, San Francisco, California, United States

Abstract

A smart Oxygen Cuvette is developed by coating the inner surface of a cuvette with oxygen sensitive thin film material. The coating is glass like sol-gel based sensor that has an embedded ruthenium compound in the glass film. The fluorescence of the ruthenium is quenched depending on the oxygen level. Ocean Optics phase fluorometer, NeoFox is used to measure this rate of fluorescence quenching and computes it for the amount of oxygen present. Multimode optical fibers are used for transportation of light from an LED source to cuvette and from cuvette to phase fluorometer. This new oxygen sensing system yields an inexpensive solution for monitoring the dissolved oxygen in samples for biological and medical applications. In addition to desktop fluorometers, smart oxygen cuvettes can be used with the Ocean Optics handheld Fluorometers, NeoFox Sport. The Smart Oxygen Cuvettes provide a resolution of 4PPB units, an accuracy of less than 5% of the reading, and 90% response in less than 10 seconds.

Citation Download Citation

Harish Dabhi, Suresh Kumar Alla, and Mahmoud R. Shahriari "Smart oxygen cuvette for optical monitoring of dissolved oxygen in biological blood samples", Proc. SPIE 7576, Reporters, Markers, Dyes, Nanoparticles, and Molecular Probes for Biomedical Applications II, 75761B (16 February 2010); https://doi.org/10.1117/12.840928

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