Green fluorescent nanodiamond conjugates and their possible applications for biosensing

Joerg Opitz; Msau Mkandawire; Mathias Sorge; Norman Rose; Martin Rudolph; Peter Krueger; Inga Hannstein; Victoria A. Lapina; Dietmar Appelhans; Wolfgang Pompe; Juergen Schreiber; Gerhard Roedel

doi:10.1117/12.860526

24 August 2010 Green fluorescent nanodiamond conjugates and their possible applications for biosensing

Joerg Opitz, Msau Mkandawire, Mathias Sorge, Norman Rose, Martin Rudolph, Peter Krueger, Inga Hannstein, Victoria A. Lapina, Dietmar Appelhans, Wolfgang Pompe, Juergen Schreiber, Gerhard Roedel

Author Affiliations +

Proceedings Volume 7759, Biosensing III; 775914 (2010) https://doi.org/10.1117/12.860526
Event: SPIE NanoScience + Engineering, 2010, San Diego, California, United States

Abstract

Various nanoparticles play a prominent role in modern biosciences and medicine. Especially fluorescent cellular biomarkers are a prospective material for diagnostics and therapy. Nevertheless, most of the available biomarkers have some drawbacks due to either physical and optical or cytotoxic properties. Here we investigated the potential of green fluorescent nanodiamonds as extra- and intracellular biomarkers for living cells. We characterized the structure of the used detonation synthesized nanodiamonds (DND) by X-ray diffraction (XRD) and the optical properties by fluorescence and infrared spectroscopy. For the extracellular attachment the nanodiamonds were functionalized by attaching antibodies that target extracellular structures such as membrane. Transfections were mediated by dendrimers, cationic liposomes and protamine sulfate. Using fluorescence microscopy, we confirmed successful extracellular binding on and transfection of the nanodiamonds into prostate cancer cells. Furthermore, nanodiamonds can be targeted selectively to intracellular structures. Therefore, nanodiamonds are a promising tool for biosensing.

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Joerg Opitz, Msau Mkandawire, Mathias Sorge, Norman Rose, Martin Rudolph, Peter Krueger, Inga Hannstein, Victoria A. Lapina, Dietmar Appelhans, Wolfgang Pompe, Juergen Schreiber, and Gerhard Roedel "Green fluorescent nanodiamond conjugates and their possible applications for biosensing", Proc. SPIE 7759, Biosensing III, 775914 (24 August 2010); https://doi.org/10.1117/12.860526

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