Efficacy of low-power laser irradiation in the prevention of D-galactose-induced senescence in human dermal fibroblasts

Chengbo Meng; Shengnan Wu; Da Xing

doi:10.1117/12.874155

17 February 2011 Efficacy of low-power laser irradiation in the prevention of D-galactose-induced senescence in human dermal fibroblasts

Chengbo Meng, Shengnan Wu, Da Xing

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Proceedings Volume 7887, Mechanisms for Low-Light Therapy VI; 78870P (2011) https://doi.org/10.1117/12.874155
Event: SPIE BiOS, 2011, San Francisco, California, United States

Abstract

Low-power laser (He-Ne) irradiation (LPLI) has been found to modulate various biological effects, especially those involved in promoting cell proliferation and metabolic regulation. However, the underlying mechanisms that LPLI prevents human cell senescence remain undefined. Herein, we devised a model enabling cell senescence using D-galactose for two days then treat with or without LPLI(< 15J/cm²), and investigated whether LPLI delays cell senescent in human dermal fibroblasts cells (HDF-a). First in this study, using SA-β-gal staining, compared with control cell we detected a lower frequency of SA-β-gal staining under the treatment of LPLI. Moreover, we found the growth rates of cell with LPLI was higher using CCK-8 analysis. Additionally, we also found LPLI induced HDF-a entered the irreversible G1 arrest measured by flow cytometry system. Therefore, LPLI may promote cell proliferation by stimulating cell-cycle progression and delay human cell senescence. Taken together, Low-power laser irradiation delay HDF-a cells senescence provides new information for the mechanisms of biological effects of LPLI.

Citation Download Citation

Chengbo Meng, Shengnan Wu, and Da Xing "Efficacy of low-power laser irradiation in the prevention of D-galactose-induced senescence in human dermal fibroblasts", Proc. SPIE 7887, Mechanisms for Low-Light Therapy VI, 78870P (17 February 2011); https://doi.org/10.1117/12.874155

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