Characterizing collagen-based materials modified by glycation: a multiphoton optical image guided spectroscopy method

Yu-Jer Hwang; Joseph Granelli; Christina Flores; Julia Lyubovitsky

doi:10.1117/12.877112

28 February 2011 Characterizing collagen-based materials modified by glycation: a multiphoton optical image guided spectroscopy method

Yu-Jer Hwang, Joseph Granelli, Christina Flores, Julia Lyubovitsky

Author Affiliations +

Proceedings Volume 7902, Imaging, Manipulation, and Analysis of Biomolecules, Cells, and Tissues IX; 79021I (2011) https://doi.org/10.1117/12.877112
Event: SPIE BiOS, 2011, San Francisco, California, United States

Abstract

In spite of the adverse ageing effects of glycation in vivo, in vitro this process is widely employed to increase stiffness and strength of tissues' and artificial scaffolds'. In-situ optical characterization methods that report on the structures within these materials could clarify the effects of glycation. We employed one-photon fluorescence and multiphoton microscopy method that combined two-photon fluorescence and second harmonic generation signals to characterize collagen hydrogels modified with glyceraldehyde, ribose and glucose. We observed an increase in the in situ fluorescence as well as structural alterations within the materials during the course of glycation.

Citation Download Citation

Yu-Jer Hwang, Joseph Granelli, Christina Flores, and Julia Lyubovitsky "Characterizing collagen-based materials modified by glycation: a multiphoton optical image guided spectroscopy method", Proc. SPIE 7902, Imaging, Manipulation, and Analysis of Biomolecules, Cells, and Tissues IX, 79021I (28 February 2011); https://doi.org/10.1117/12.877112

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