Comparison of algorithms for the trapping of single molecules in an electrostatic corral

Jörg C. Woehl

doi:10.1117/12.875508

22 February 2011 Comparison of algorithms for the trapping of single molecules in an electrostatic corral

Jörg C. Woehl

Proceedings Volume 7905, Single Molecule Spectroscopy and Imaging IV; 79050A (2011) https://doi.org/10.1117/12.875508
Event: SPIE BiOS, 2011, San Francisco, California, United States

Abstract

The corral trap is a novel tool for the solution-based trapping of single fluorescent molecules and other nanoparticles with great potential for applications in ultrasensitive biomedical analysis at the single molecule level. This article describes a general approach for automated trapping based on particle detection in two regions of interest close to the corral trap: the doormat region, which triggers the "opening" of the corral trap, and the trigger region, which triggers the "closing" of the trap. Three different algorithms for rapid particle detection in these two regions are presented, and their execution time is evaluated for different experimental scenarios.

Citation Download Citation

Jörg C. Woehl "Comparison of algorithms for the trapping of single molecules in an electrostatic corral", Proc. SPIE 7905, Single Molecule Spectroscopy and Imaging IV, 79050A (22 February 2011); https://doi.org/10.1117/12.875508

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