In situ formation of microstructures near live cells using spatially structured near-infrared laser microbeam

Ninad Ingle; Ling Gu; Samarendra K. Mohanty

doi:10.1117/12.876009

11 February 2011 In Situ formation of microstructures near live cells using spatially structured near-infrared laser microbeam

Ninad Ingle, Ling Gu, Samarendra K. Mohanty

Author Affiliations +

Proceedings Volume 7908, Nanoscale Imaging, Sensing, and Actuation for Biomedical Applications VIII; 79080I (2011) https://doi.org/10.1117/12.876009
Event: SPIE BiOS, 2011, San Francisco, California, United States

Abstract

Here, we report in situ formation of microstructures from the regular constituents of culture media near live cells using spatially-structured near infrared (NIR) laser beam. Irradiation with the continuous wave (cw) NIR laser microbeam for few seconds onto the regular cell culture media containing fetal bovine serum resulted in accumulation of dense material inside the media as evidenced by phase contrast microscopy. The time to form the phase dense material was found to depend on the laser beam power. Switching off the laser beam led to diffusion of phase dark material. However, the proteins could be stitched together by use of carbon nanoparticles and continuous wave (cw) Ti: Sapphire laser beam. Further, by use of spatially-structured beam profiles different structures near live cells could be formed. The microfabricated structure could be held by the Gravito-optical trap and repositioned by movement of the sample stage. Orientation of these microstructures was achieved by rotating the elliptical laser beam profile. Thus, multiple microstructures were formed and organized near live cells. This method would enable study of response of cells/axons to the immediate physical hindrance provided by such structure formation and also eliminate the biocompatibility requirement posed on artificial microstructure materials.

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Ninad Ingle, Ling Gu, and Samarendra K. Mohanty "In Situ formation of microstructures near live cells using spatially structured near-infrared laser microbeam", Proc. SPIE 7908, Nanoscale Imaging, Sensing, and Actuation for Biomedical Applications VIII, 79080I (11 February 2011); https://doi.org/10.1117/12.876009

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KEYWORDS

Continuous wave operation

Proteins

Molecules

Near infrared

Microscopes

Objectives

Microfabrication

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