Free-flow electrophoresis with electrode-less injection molded chips

Stefan Köhler; Holger Becker; Volker Beushausen; Wilhelm Hüttner; Hainer Wackerbarth; Erik Beckert; Steffen Howitz; Detlev Belder

doi:10.1117/12.874713

14 February 2011 Free-flow electrophoresis with electrode-less injection molded chips

Stefan Köhler, Holger Becker, Volker Beushausen, Wilhelm Hüttner, Hainer Wackerbarth, Erik Beckert, Steffen Howitz, Detlev Belder

Author Affiliations +

Proceedings Volume 7929, Microfluidics, BioMEMS, and Medical Microsystems IX; 79290O (2011) https://doi.org/10.1117/12.874713
Event: SPIE MOEMS-MEMS, 2011, San Francisco, California, United States

Abstract

In this work we present the first approach towards low-cost free-flow electrophoresis (FFE) devices utilizing injection molding as a microfabrication process which has the potential to manufacture FFE chips at a cost which make their use commercially viable. This is achieved by realizing a new straightforward micro free-flow electrophoresis (μFFE) design ensuring both, bubble free electrophoretic separation and effective electrical connection by implementing miniaturized partitioning bars. This creates a defined open gap of 20 μm in height and 500 μm in width between separation zone and electrode channels. The thermoplastic μFFE chips are ready to use, there is no need for a subsequent labor-intensive implementation of membranes or salt bridges to separate the electrode channels from the separation zone.

Citation Download Citation

Stefan Köhler, Holger Becker, Volker Beushausen, Wilhelm Hüttner, Hainer Wackerbarth, Erik Beckert, Steffen Howitz, and Detlev Belder "Free-flow electrophoresis with electrode-less injection molded chips", Proc. SPIE 7929, Microfluidics, BioMEMS, and Medical Microsystems IX, 79290O (14 February 2011); https://doi.org/10.1117/12.874713

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