Robust detection of peak signals for lateral flow immunoassays

Jongwon Kim; Jong Dae Kim; Kie Bong Nahm; Eui Yul Choi; Geumyoung Lee

doi:10.1117/12.876260

14 February 2011 Robust detection of peak signals for lateral flow immunoassays

Jongwon Kim, Jong Dae Kim, Kie Bong Nahm, Eui Yul Choi, Geumyoung Lee

Proceedings Volume 7929, Microfluidics, BioMEMS, and Medical Microsystems IX; 792910 (2011) https://doi.org/10.1117/12.876260
Event: SPIE MOEMS-MEMS, 2011, San Francisco, California, United States

Abstract

Template matching method is presented to identify the peaks from the scanned signals of lateral flow immunoassay strips. The template is composed of two pulses separated by the distance of the control and the target ligand line in the assay, and is convolved with the scanned signal to deliver the maximum at the center of the two peaks. The peak regions were identified with the predefined distances from the center. Glycosylated haemoglobin immunoassay strips and fluorescent strip readers from Boditechmed Inc. were tested to estimate the lot and reader variations of the concentration measurands. The results showed the robustness of the propose method.

Citation Download Citation

Jongwon Kim, Jong Dae Kim, Kie Bong Nahm, Eui Yul Choi, and Geumyoung Lee "Robust detection of peak signals for lateral flow immunoassays", Proc. SPIE 7929, Microfluidics, BioMEMS, and Medical Microsystems IX, 792910 (14 February 2011); https://doi.org/10.1117/12.876260

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