Adaptive optics wide-field microscope corrections using a MEMS DM and Shack-Hartmann wavefront sensor

Oscar Azucena; Xiaodong Tao; Justin Crest; Shaila Kotadia; William Sullivan; Donald Gavel; Marc Reinig; Scot Olivier; Joel Kubby

doi:10.1117/12.876439

14 February 2011 Adaptive optics wide-field microscope corrections using a MEMS DM and Shack-Hartmann wavefront sensor

Oscar Azucena, Xiaodong Tao, Justin Crest, Shaila Kotadia, William Sullivan, Donald Gavel, Marc Reinig, Scot Olivier, Joel Kubby

Author Affiliations +

Proceedings Volume 7931, MEMS Adaptive Optics V; 79310J (2011) https://doi.org/10.1117/12.876439
Event: SPIE MOEMS-MEMS, 2011, San Francisco, California, United States

Abstract

We demonstrated the used of an adaptive optic system in biological imaging to improve the imaging characteristics of a wide field microscope. A crimson red fluorescent bead emitting light at 650 nm was used together with a Shack-Hartmann wavefront sensor and deformable mirror to compensate for the aberrations introduce by a Drosophila embryo. The measurement and correction at one wavelength improves the resolving power at a different wavelength, enabling the structure of the sample to be resolved (510 nm). The use of the crimson beads allow for less photobleaching to be done to the science object of the embryo, in this case our GFP model (green fluorescent beads), and allows for the science object and wavefront reference to be spectrally separated. The spectral separation allows for single points sources to be used for wavefront measurements, which is a necessary condition for the Shack-Hartmann Wavefront sensor operation.

Citation Download Citation

Oscar Azucena, Xiaodong Tao, Justin Crest, Shaila Kotadia, William Sullivan, Donald Gavel, Marc Reinig, Scot Olivier, and Joel Kubby "Adaptive optics wide-field microscope corrections using a MEMS DM and Shack-Hartmann wavefront sensor", Proc. SPIE 7931, MEMS Adaptive Optics V, 79310J (14 February 2011); https://doi.org/10.1117/12.876439

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