Two-channel microfluidic CARS: experimental quantification of pure vibrational contrast in CARS images

G. Bergner; T. Henkel; D. Akimov; B. Dietzek; S. Schlücker; H. Bartelt; J. Popp

doi:10.1117/12.889925

10 June 2011 Two-channel microfluidic CARS: experimental quantification of pure vibrational contrast in CARS images

G. Bergner, T. Henkel, D. Akimov, B. Dietzek, S. Schlücker, H. Bartelt, J. Popp

Proceedings Volume 8087, Clinical and Biomedical Spectroscopy and Imaging II; 80871U (2011) https://doi.org/10.1117/12.889925
Event: European Conferences on Biomedical Optics, 2011, Munich, Germany

Abstract

The combination of linear and nonlinear Raman microspectroscopy has been established to be a powerful tool for biomedical diagnostics. In this contribution we discuss our recent approaches towards CARS (coherent anti-Stokes Raman scattering) based quantification of analytes, which is generally complicated by the CARS-signal strength dependence on the square of the molecular concentration and on the interplay between a molecular-specific vibrational signal and a nonresonant contribution in the signal generation. Due to these complications the quantification of analytes presents a major challenge in CARS microscopy. Here we discuss two recently developed approaches, i.e. on the one hand a simplified setup for coherent anti-Stokes Raman scattering (CARS) microscopy, which allows for recording CARS images with 30 cm^-1 excitation bandwidth for probing Raman bands between 500 and 900 cm^-1 with minimal requirements for alignment. This experimental arrangement is based on electronic switching between CARS images recorded at different Raman resonances by combining a photonic crystal fiber (PCF) as broad-band light source and an acoustooptical programmable dispersive filter (AOPDF) as tunable wavelength filter. On the other hand, we discuss how the introduction of carbon-deuterium (C-D) bonds into drug compounds constitutes a non-invasive labeling approach that allows for higher intrinsic CARS contrast to be obtained. The quantitative detection of C-deuterated drugs by Raman microspectroscopy and CARS microscopy is examined. Concentration-dependent studies on drugs with aliphatic and aromatic C-D moieties were performed in a two-channel microfluidic chip, using the corresponding non-deuterated (C-H) isotopomers as an internal reference.

Citation Download Citation

G. Bergner, T. Henkel, D. Akimov, B. Dietzek, S. Schlücker, H. Bartelt, and J. Popp "Two-channel microfluidic CARS: experimental quantification of pure vibrational contrast in CARS images", Proc. SPIE 8087, Clinical and Biomedical Spectroscopy and Imaging II, 80871U (10 June 2011); https://doi.org/10.1117/12.889925

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