Heavy metal stress detection and monitoring via LED-induced chlorophyll fluorescence analysis of Zea mays L. seedlings aimed at polluted soil phytoremediation

Artur S. Gouveia-Neto; Elias A. Silva Jr.; Airon José da Silva; Clístenes W. A. do Nascimento

doi:10.1117/12.905456

9 February 2012 Heavy metal stress detection and monitoring via LED-induced chlorophyll fluorescence analysis of Zea mays L. seedlings aimed at polluted soil phytoremediation

Artur S. Gouveia-Neto, Elias A. Silva Jr., Airon José da Silva, Clístenes W. A. do Nascimento

Author Affiliations +

Proceedings Volume 8225, Imaging, Manipulation, and Analysis of Biomolecules, Cells, and Tissues X; 822505 (2012) https://doi.org/10.1117/12.905456
Event: SPIE BiOS, 2012, San Francisco, California, United States

Abstract

Chlorophyll fluorescence spectroscopy is employed to detect and study the time evolution of metal stress of Zea mays L. seedlings aiming polluted soil phytoremediation. The chlorophyll fluorescence spectra of intact leaves are analyzed using 405 nm LED excitation. Red (Fr) and far-red (FFr) emissions around 685 nm and 735 nm, respectively, are examined as a function of the heavy metal concentration. The fluorescence ratio Fr/FFr was employed to monitor the effect of heavy metal upon the physiological state of the plants before signs of visual stress became apparent. The chlorophyll fluorescence analysis permitted detection and evaluation of the damage caused by heavy metal soil contamination in the early stages of the plants growing process, which is not feasible using conventional in vitro spectral analysis.

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Artur S. Gouveia-Neto, Elias A. Silva Jr., Airon José da Silva, and Clístenes W. A. do Nascimento "Heavy metal stress detection and monitoring via LED-induced chlorophyll fluorescence analysis of Zea mays L. seedlings aimed at polluted soil phytoremediation", Proc. SPIE 8225, Imaging, Manipulation, and Analysis of Biomolecules, Cells, and Tissues X, 822505 (9 February 2012); https://doi.org/10.1117/12.905456

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