MEM-FLIM: all-solid-state camera for fluorescence lifetime imaging

Qiaole Zhao; Ian Ted Young; Ben Schelen; Raymond Schouten; Rein van den Oever; René Leenen; Harry van Kuijk; Inge Peters; Frank Polderdijk; Jan Bosiers; Kees Jalink; Sander de Jong; Bert van Geest; Karel Stoop

doi:10.1117/12.905345

9 February 2012 MEM-FLIM: all-solid-state camera for fluorescence lifetime imaging

Qiaole Zhao, Ian Ted Young, Ben Schelen, Raymond Schouten, Rein van den Oever, René Leenen, Harry van Kuijk, Inge Peters, Frank Polderdijk, Jan Bosiers, Kees Jalink, Sander de Jong, Bert van Geest, Karel Stoop

Author Affiliations +

Proceedings Volume 8225, Imaging, Manipulation, and Analysis of Biomolecules, Cells, and Tissues X; 82250N (2012) https://doi.org/10.1117/12.905345
Event: SPIE BiOS, 2012, San Francisco, California, United States

Abstract

We have built an all-solid-state camera which is directly modulated at the pixel level for frequency domain fluorescence lifetime imaging microscopy (FLIM) measurement. This novel camera eliminates the need for an image intensifier through the use of an application-specific CCD design, which is being used in a frequency domain FLIM system. The first stage of evaluation for the camera has been carried out. Camera characteristics such as noise distribution, dark current influence, camera gain, sampling density, sensitivity, linearity of photometric response, and contrast modulation transfer function have been studied through experiments. We are able to do lifetime measurement using MEM-FLIM cameras for various objects, e.g. fluorescence plastic test slides, fluorescein solution, fixed GFP cells, and GFP - Actin stained live cells.

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Qiaole Zhao, Ian Ted Young, Ben Schelen, Raymond Schouten, Rein van den Oever, René Leenen, Harry van Kuijk, Inge Peters, Frank Polderdijk, Jan Bosiers, Kees Jalink, Sander de Jong, Bert van Geest, and Karel Stoop "MEM-FLIM: all-solid-state camera for fluorescence lifetime imaging", Proc. SPIE 8225, Imaging, Manipulation, and Analysis of Biomolecules, Cells, and Tissues X, 82250N (9 February 2012); https://doi.org/10.1117/12.905345

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