Fluorescent nanodiamonds as highly stable biomarker for endotoxin verification

Thorsten Bergmann; Jan Michael Burg; Maria Lilholt; Ulf Maeder; Sebastian Beer; Denise Salzig; Mehrdad Ebrahimi; Peter Czermak; Martin Fiebich

doi:10.1117/12.909251

2 February 2012 Fluorescent nanodiamonds as highly stable biomarker for endotoxin verification

Thorsten Bergmann, Jan Michael Burg, Maria Lilholt, Ulf Maeder, Sebastian Beer, Denise Salzig, Mehrdad Ebrahimi, Peter Czermak, Martin Fiebich

Author Affiliations +

Proceedings Volume 8233, Reporters, Markers, Dyes, Nanoparticles, and Molecular Probes for Biomedical Applications IV; 82331H (2012) https://doi.org/10.1117/12.909251
Event: SPIE BiOS, 2012, San Francisco, California, United States

Abstract

Fluorescent nanodiamonds (ND) provide advantageous properties as a fluorescent biomarker for in vitro and in vivo studies. The maximum fluorescence occurs around 700 nm, they do not show photobleaching or blinking and seem to be nontoxic. After a pretreatment with strong acid fluorescent ND can be functionalized and coupled to endotoxin. Endotoxin is a decay product of bacteria and causes strong immune reactions. Therefore endotoxin has to be removed for most applications. An effective removal procedure is membrane filtration. The endotoxin, coupled to fluorescent ND can be visualized by using confocal microscopy which allows the investigation of the separation mechanisms of the filtration process within the membranes.

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Thorsten Bergmann, Jan Michael Burg, Maria Lilholt, Ulf Maeder, Sebastian Beer, Denise Salzig, Mehrdad Ebrahimi, Peter Czermak, and Martin Fiebich "Fluorescent nanodiamonds as highly stable biomarker for endotoxin verification", Proc. SPIE 8233, Reporters, Markers, Dyes, Nanoparticles, and Molecular Probes for Biomedical Applications IV, 82331H (2 February 2012); https://doi.org/10.1117/12.909251

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KEYWORDS

Neodymium

Luminescence

Particles

Confocal microscopy

Visualization

Diamond

Proteins

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