Nano particles insertion into individual mammalian cells using optical tweezers

Muhammad Waleed; Jung-Dae Kim; Yong-Gu Lee

doi:10.1117/12.912056

30 January 2012 Nano particles insertion into individual mammalian cells using optical tweezers

Muhammad Waleed, Jung-Dae Kim, Yong-Gu Lee

Proceedings Volume 8247, Frontiers in Ultrafast Optics: Biomedical, Scientific, and Industrial Applications XII; 824706 (2012) https://doi.org/10.1117/12.912056
Event: SPIE LASE, 2012, San Francisco, California, United States

Abstract

Transfection is the process of introducing DNA into cells so that the introduced DNA will function and produce proteins. This technique is useful to study the function of various DNA sequences and in the future may lead to gene therapy for curing genetic diseases. Currently, a number of techniques are available for both population and individual cells transfection. Although individual cells transfection is less commonly used than the population transfection, it has benefits because it allows controlled single cell analysis. In this paper, we present a new laser assisted transfection method for individual cells. In this technique, two lasers are used to perform the transfection procedure and third laser is used to detect the position of DNA coated nanoparticle which is inserted in the cell. This technique has relatively high transfection efficiency and good post-transfection cell viability.

Citation Download Citation

Muhammad Waleed, Jung-Dae Kim, and Yong-Gu Lee "Nano particles insertion into individual mammalian cells using optical tweezers", Proc. SPIE 8247, Frontiers in Ultrafast Optics: Biomedical, Scientific, and Industrial Applications XII, 824706 (30 January 2012); https://doi.org/10.1117/12.912056

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