Highly accurate measurement of varying drug dosage for real-time analysis of chemomechanical response of cardiomyocytes

Avneet Bajwa; Behraad Bahreyni; Ash M. Parameswaran

doi:10.1117/12.907105

14 February 2012 Highly accurate measurement of varying drug dosage for real-time analysis of chemomechanical response of cardiomyocytes

Avneet Bajwa, Behraad Bahreyni, Ash M. Parameswaran

Author Affiliations +

Proceedings Volume 8251, Microfluidics, BioMEMS, and Medical Microsystems X; 82510N (2012) https://doi.org/10.1117/12.907105
Event: SPIE MOEMS-MEMS, 2012, San Francisco, California, United States

Abstract

A recent advancement in the study of drug development for cardiovascular diseases is based on measuring the mechanical response of a single cardiomyocyte to various drug concentrations. This method requires delivering a specific dose of the drug over a short period of time while measuring the forces exerted by a cell that is kept inside a microchamber. However, the exact drug dosage is difficult to control for rapid variations in drug concentration, which hinders the accuracy of the measurements. This paper reports a highly sensitive technique for accurate and real-time measurement of minute variations in drug concentration. The fluid electrical conductivity is monitored using an array of electrodes along a micro-channel that eventually leads to the microchamber where the cardiomyocyte is placed. The microfluidic setup is fabricated through bonding of a moulded Polydimethylsiloxane (PDMS) layer to a glass substrate with patterned gold electrodes. The real-time differential measurements let us measure the local drug concentration with accuracies of better than 10pMol/mL. By using the data from all of the array electrodes, the profile of the drug plug as it travels along the microchannel from the injection point to the cell location can be derived with high precision. The multidomain numerical simulations of the microfluidic setup are in line with the measured experimental data. Our technique can be easily integrated into many existing and new designs thus providing a robust approach for label-free measurement of fluid properties in cell viability studies.

Citation Download Citation

Avneet Bajwa, Behraad Bahreyni, and Ash M. Parameswaran "Highly accurate measurement of varying drug dosage for real-time analysis of chemomechanical response of cardiomyocytes", Proc. SPIE 8251, Microfluidics, BioMEMS, and Medical Microsystems X, 82510N (14 February 2012); https://doi.org/10.1117/12.907105

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KEYWORDS

Electrodes

Microfluidics

Diffusion

3D modeling

Time metrology

Drug development

Ions

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