Inhomogeneity in optical properties of rat brain: a study for LLLT dosimetry

Marcelo V. P. Sousa; Renato Prates; Ilka T. Kato; Caetano P. Sabino; Tania M. Yoshimura; Luis C. Suzuki; Ana C. Magalhães; Elisabeth M. Yoshimura; Martha S. Ribeiro

doi:10.1117/12.2002836

5 March 2013 Inhomogeneity in optical properties of rat brain: a study for LLLT dosimetry

Marcelo V. P. Sousa, Renato Prates, Ilka T. Kato, Caetano P. Sabino, Tania M. Yoshimura, Luis C. Suzuki, Ana C. Magalhães, Elisabeth M. Yoshimura, Martha S. Ribeiro

Author Affiliations +

Proceedings Volume 8569, Mechanisms for Low-Light Therapy VIII; 856905 (2013) https://doi.org/10.1117/12.2002836
Event: SPIE BiOS, 2013, San Francisco, California, United States

Abstract

Over the last few years, low-level light therapy (LLLT) has shown an incredible suitability for a wide range of applications for central nervous system (CNS) related diseases. In this therapeutic modality light dosimetry is extremely critical so the study of light propagation through the CNS organs is of great importance. To better understand how light intensity is delivered to the most relevant neural sites we evaluated optical transmission through slices of rat brain point by point. We experimented red (λ = 660 nm) and near infrared (λ = 808 nm) diode laser light analyzing the light penetration and distribution in the whole brain. A fresh Wistar rat (Rattus novergicus) brain was cut in sagittal slices and illuminated with a broad light beam. A high-resolution digital camera was employed to acquire data of transmitted light. Spatial profiles of the light transmitted through the sample were obtained from the images. Peaks and valleys in the profiles show sites where light was less or more attenuated. The peak intensities provide information about total attenuation and the peak widths are correlated to the scattering coefficient at that individual portion of the sample. The outcomes of this study provide remarkable information for LLLT dose-dependent studies involving CNS and highlight the importance of LLLT dosimetry in CNS organs for large range of applications in animal and human diseases.

Citation Download Citation

Marcelo V. P. Sousa, Renato Prates, Ilka T. Kato, Caetano P. Sabino, Tania M. Yoshimura, Luis C. Suzuki, Ana C. Magalhães, Elisabeth M. Yoshimura, and Martha S. Ribeiro "Inhomogeneity in optical properties of rat brain: a study for LLLT dosimetry", Proc. SPIE 8569, Mechanisms for Low-Light Therapy VIII, 856905 (5 March 2013); https://doi.org/10.1117/12.2002836

ACCESS THE FULL ARTICLE

INSTITUTIONAL
Select your institution to access the SPIE Digital Library.

SELECT YOUR INSTITUTION

PERSONAL
Sign in with your SPIE account to access your personal subscriptions or to use specific features such as save to my library, sign up for alerts, save searches, etc.

PERSONAL SIGN IN

No SPIE Account? Create one

PURCHASE THIS CONTENT

SUBSCRIBE TO DIGITAL LIBRARY

50 downloads per 1-year subscription

Members: $195

Non-members: $335 ADD TO CART

25 downloads per 1 - year subscription

Members: $145

Non-members: $250 ADD TO CART

PURCHASE SINGLE ARTICLE

Includes PDF, HTML & Video, when available