Single-molecule imaging can be achieved in live obligate anaerobic bacteria

Krishanthi S. Karunatilaka; Ben R. Coupland; Elizabeth A. Cameron; Eric C. Martens; Nicole K. Koropatkin; Julie S. Biteen

doi:10.1117/12.2004204

22 February 2013 Single-molecule imaging can be achieved in live obligate anaerobic bacteria

Krishanthi S. Karunatilaka, Ben R. Coupland, Elizabeth A. Cameron, Eric C. Martens, Nicole K. Koropatkin, Julie S. Biteen

Author Affiliations +

Proceedings Volume 8590, Single Molecule Spectroscopy and Superresolution Imaging VI; 85900K (2013) https://doi.org/10.1117/12.2004204
Event: SPIE BiOS, 2013, San Francisco, California, United States

Abstract

Single-molecule fluorescence (SMF) permits imaging with nanometer-scale resolution. This technique is particularly useful for cellular imaging as it provides a non-invasive, minimally perturbative means to examine macromolecular localization and dynamics, even in live cells. Here, we demonstrate that nanometer-scale SMF imaging can be extended to a new category of experiments: intracellular imaging of live, obligate anaerobic cells on the benchtop. We investigate the starch-utilization system (Sus) proteins in the gut symbiont Bacteroides thetaiotaomicron and discuss three different labels that we implemented to detect these proteins: fluorescent proteins, the tetracysteine-based FlAsH tag, and the enzymatic HaloTag.

Citation Download Citation

Krishanthi S. Karunatilaka, Ben R. Coupland, Elizabeth A. Cameron, Eric C. Martens, Nicole K. Koropatkin, and Julie S. Biteen "Single-molecule imaging can be achieved in live obligate anaerobic bacteria", Proc. SPIE 8590, Single Molecule Spectroscopy and Superresolution Imaging VI, 85900K (22 February 2013); https://doi.org/10.1117/12.2004204

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