A new method of real-time detection of pH based on polyaniline-coated tapered optical fiber

Yanli Hu; Wei Tao; Yubing Bi; Hui Zhao

doi:10.1117/12.2021048

22 June 2013 A new method of real-time detection of pH based on polyaniline-coated tapered optical fiber

Yanli Hu, Wei Tao, Yubing Bi, Hui Zhao

Proceedings Volume 8769, International Conference on Optics in Precision Engineering and Nanotechnology (icOPEN2013); 87692O (2013) https://doi.org/10.1117/12.2021048
Event: International Conference on Optics in Precision Engineering and Nanotechnology (icOPEN2013), 2013, Singapore, Singapore

Abstract

It is very important to monitor and control pH during cell and tissue culture. On-line pH monitoring provides valuable information on cell metabolic processes and living environment. A novel simple method to real-time measure pH during cell and tissue culture has been experimentally demonstrated using a tapered optical fiber coated with polyaniline. The fiber is tapered to produce the leaking mode in the sensing film. The absorption coefficient and the refractive index of the polyaniline film will vary with different pH values and resultantly change the optical spectral responses. The optical power droped with the increase of the pH at 1042nm. Such a device is sensitive to pH allowing the determination of pH values ranging from 5 to 11 and the resolution of the order of 0.03. Comparing to the conventional pH glass electrode, this optical measurement has smaller size, faster response and can avoid the contamination of the cell and tissue culture fluid.

Citation Download Citation

Yanli Hu, Wei Tao, Yubing Bi, and Hui Zhao "A new method of real-time detection of pH based on polyaniline-coated tapered optical fiber", Proc. SPIE 8769, International Conference on Optics in Precision Engineering and Nanotechnology (icOPEN2013), 87692O (22 June 2013); https://doi.org/10.1117/12.2021048

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