High resolution quantitative phase imaging in digital holographic microscopy by modulated object illumination with an electrically focusable lens

Björn Kemper; Robin Schubert; Angelika Vollmer

doi:10.1117/12.2032497

17 June 2013 High resolution quantitative phase imaging in digital holographic microscopy by modulated object illumination with an electrically focusable lens

Björn Kemper, Robin Schubert, Angelika Vollmer

Author Affiliations +

Proceedings Volume 8797, Advanced Microscopy Techniques III; 87970A (2013) https://doi.org/10.1117/12.2032497
Event: European Conferences on Biomedical Optics, 2013, Munich, Germany

Abstract

In digital holographic microscopy (DHM), scattering patterns that are induced by coherent laser light affect the resolution for the detection of optical path length changes. We present a simple and efficient approach for the reduction of coherent disturbances in quantitative phase imaging in self-interference DHM that is based on amplitude and phase modulation of the sample illumination. The performance of the method for quantitative phase imaging is characterized and the application for quantitative analysis of living cells is illustrated.

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Björn Kemper, Robin Schubert, and Angelika Vollmer "High resolution quantitative phase imaging in digital holographic microscopy by modulated object illumination with an electrically focusable lens", Proc. SPIE 8797, Advanced Microscopy Techniques III, 87970A (17 June 2013); https://doi.org/10.1117/12.2032497

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