Relative deformability of red blood cells in sickle cell trait and sickle cell anemia by trapping and dragging

Rance Solomon; James Cooper; Gabriel Welker; Elaura Aguilar; Brooke Flanagan; Chelsey Pennycuff; David Scott; Anthony Farone; Mary Farone; Daniel Erenso; Robert Mushi; Maria del Pilar Aguinaga

doi:10.1117/12.2032591

24 June 2013 Relative deformability of red blood cells in sickle cell trait and sickle cell anemia by trapping and dragging

Rance Solomon, James Cooper, Gabriel Welker, Elaura Aguilar, Brooke Flanagan, Chelsey Pennycuff, David Scott, Anthony Farone, Mary Farone, Daniel Erenso, Robert Mushi, Maria del Pilar Aguinaga

Author Affiliations +

Proceedings Volume 8803, Medical Laser Applications and Laser-Tissue Interactions VI; 880307 (2013) https://doi.org/10.1117/12.2032591
Event: European Conferences on Biomedical Optics, 2013, Munich, Germany

Abstract

Genetic mutation of the β-globin gene or inheritance of this mutated gene changes the chemical composition of the oxygen-carrying hemoglobin molecule that could lead to either the heterozygote genotype, resulting in sickle cell trait (SCT), or the homozygote genotype, resulting in sickle cell anemia (SCA). These mutations could affect the reversible elastic deformations of the red blood cells (RBCs) which are vital for biological functions. We have investigated this effect by studying the differences in the deformability of RBCs from blood samples of an individual with SCT and an untreated patient with SCA along with hemoglobin quantitation of each blood sample. Infrared 1064 nm laser trap force along with drag shear force are used to induce deformation in the RBCs. Ultra2-High Performance Liquid Chromatography (UHPLC) is used for the hemoglobin quantitation.

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Rance Solomon, James Cooper, Gabriel Welker, Elaura Aguilar, Brooke Flanagan, Chelsey Pennycuff, David Scott, Anthony Farone, Mary Farone, Daniel Erenso, Robert Mushi, and Maria del Pilar Aguinaga "Relative deformability of red blood cells in sickle cell trait and sickle cell anemia by trapping and dragging", Proc. SPIE 8803, Medical Laser Applications and Laser-Tissue Interactions VI, 880307 (24 June 2013); https://doi.org/10.1117/12.2032591

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KEYWORDS

Blood

Microscopes

Biomedical optics

Genetics

Infrared lasers

Picosecond phenomena

Chromatography

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