An optical platform for the production, trapping, manipulation and
visualization of ultra-low interfacial tension emulsion droplets

Alexander L. Hargreaves; Andrew K. Kirby; Colin D. Bain; Gordon D. Love; Guido Bolognesi; Oscar Ces; Mark Neil; Andrew D. Ward

doi:10.1117/12.2027105

12 September 2013 An optical platform for the production, trapping, manipulation and visualization of ultra-low interfacial tension emulsion droplets

Alexander L. Hargreaves, Andrew K. Kirby, Colin D. Bain, Gordon D. Love, Guido Bolognesi, Oscar Ces, Mark Neil, Andrew D. Ward

Author Affiliations +

Proceedings Volume 8810, Optical Trapping and Optical Micromanipulation X; 88101W (2013) https://doi.org/10.1117/12.2027105
Event: SPIE NanoScience + Engineering, 2013, San Diego, California, United States

Abstract

We discuss the design, implementation and performance of a novel platform for the production and optical control of ultra-low interfacial tension droplets in the 1-10 micron regime. A custom-designed, integrated microfluidic system allows the production of oil-in-water emulsion droplets of controllable size. This provides an optimised physical platform in which individual droplets are selected, trapped and shaped by holographic optical tweezers (HOTs) via extended optical landscaping. The 3D structure of the shaped droplet is interrogated by a combination of conventional brightfield imaging and fluorescent structured-illumination sectioning. We detail the problems and limitations of closed-loop holographic control of droplet shape.

Citation Download Citation

Alexander L. Hargreaves, Andrew K. Kirby, Colin D. Bain, Gordon D. Love, Guido Bolognesi, Oscar Ces, Mark Neil, and Andrew D. Ward "An optical platform for the production, trapping, manipulation and visualization of ultra-low interfacial tension emulsion droplets", Proc. SPIE 8810, Optical Trapping and Optical Micromanipulation X, 88101W (12 September 2013); https://doi.org/10.1117/12.2027105

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