3D manipulation and visualization of in-vitro cells by optical tweezers and digital holographic microscopy

F. Merola; L. Miccio; P. Memmolo; G. Di Caprio; G. Coppola; P. Netti; P. Ferraro

doi:10.1117/12.2039423

4 March 2014 3D manipulation and visualization of in-vitro cells by optical tweezers and digital holographic microscopy

F. Merola, L. Miccio, P. Memmolo, G. Di Caprio, G. Coppola, P. Netti, P. Ferraro

Proceedings Volume 8947, Imaging, Manipulation, and Analysis of Biomolecules, Cells, and Tissues XII; 89471A (2014) https://doi.org/10.1117/12.2039423
Event: SPIE BiOS, 2014, San Francisco, California, United States

Abstract

We present the possibility to trap cells (mouse fibroblasts, bovine spermatozoa and diatoms), to manage their position and to induce rotation, by using optical tweezers. The aim is to place them in desired positions, in order to record holographic images in a microscope configuration. Then we are able to recover the 3D shape and to calculate the biovolume of the cells starting from the reconstructed quantitative phase maps (QPMs).

Citation Download Citation

F. Merola, L. Miccio, P. Memmolo, G. Di Caprio, G. Coppola, P. Netti, and P. Ferraro "3D manipulation and visualization of in-vitro cells by optical tweezers and digital holographic microscopy", Proc. SPIE 8947, Imaging, Manipulation, and Analysis of Biomolecules, Cells, and Tissues XII, 89471A (4 March 2014); https://doi.org/10.1117/12.2039423

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