Lens-free digital in-line holographic imaging for wide field-of-view, high-resolution and real-time monitoring of complex microscopic objects

Richard Stahl; Geert Vanmeerbeeck; Gauthier Lafruit; Roeland Huys; Veerle Reumers; Andy Lambrechts; Chao-Kang Liao; Chin-Chun Hsiao; Masayuki Yashiro; Masashi Takemoto; Tomohisa Nagata; Shinichi Gomi; Kunitada Hatabayashi; Yasuhiro Oshima; Shigenori Ozaki; Naoki Nishishita; Shin Kawamata

doi:10.1117/12.2037619

4 March 2014 Lens-free digital in-line holographic imaging for wide field-of-view, high-resolution and real-time monitoring of complex microscopic objects

Richard Stahl, Geert Vanmeerbeeck, Gauthier Lafruit, Roeland Huys, Veerle Reumers, Andy Lambrechts, Chao-Kang Liao, Chin-Chun Hsiao, Masayuki Yashiro, Masashi Takemoto, Tomohisa Nagata, Shinichi Gomi, Kunitada Hatabayashi, Yasuhiro Oshima, Shigenori Ozaki, Naoki Nishishita, Shin Kawamata

Author Affiliations +

Proceedings Volume 8947, Imaging, Manipulation, and Analysis of Biomolecules, Cells, and Tissues XII; 89471F (2014) https://doi.org/10.1117/12.2037619
Event: SPIE BiOS, 2014, San Francisco, California, United States

Abstract

Lens-free inline Holographic Microscopy (LHM) holds great promise for biomedical and industrial applications thanks to its conceptual simplicity. However, the challenge lies in achieving an image quality comparable to conventional microscopes. We demonstrate a high-throughput LHM system that is able to resolve 1.23μm-thin lines on a standard USAF 1951 test target with 1.67μm pixels at the full field-of-view (>29mm²). The system is based on a unique multiwavelength iterative-phase-retrieval method, using customized hardware and real-time post-processing software. We have evaluated our system in experiments ranging from single-cell inspection to in-vitro imaging of stem-cell colonies.

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Richard Stahl, Geert Vanmeerbeeck, Gauthier Lafruit, Roeland Huys, Veerle Reumers, Andy Lambrechts, Chao-Kang Liao, Chin-Chun Hsiao, Masayuki Yashiro, Masashi Takemoto, Tomohisa Nagata, Shinichi Gomi, Kunitada Hatabayashi, Yasuhiro Oshima, Shigenori Ozaki, Naoki Nishishita, and Shin Kawamata "Lens-free digital in-line holographic imaging for wide field-of-view, high-resolution and real-time monitoring of complex microscopic objects", Proc. SPIE 8947, Imaging, Manipulation, and Analysis of Biomolecules, Cells, and Tissues XII, 89471F (4 March 2014); https://doi.org/10.1117/12.2037619

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