Adaptive optics for in vivo two-photon calcium imaging of neuronal networks

Serge Meimon; Jean-Marc Conan; Laurent M. Mugnier; Vincent Michau; Rosa Cossart; Arnaud Malvache

doi:10.1117/12.2044052

7 March 2014 Adaptive optics for in vivo two-photon calcium imaging of neuronal networks

Serge Meimon, Jean-Marc Conan, Laurent M. Mugnier, Vincent Michau, Rosa Cossart, Arnaud Malvache

Proceedings Volume 8978, MEMS Adaptive Optics VIII; 89780C (2014) https://doi.org/10.1117/12.2044052
Event: SPIE MOEMS-MEMS, 2014, San Francisco, California, United States

Abstract

The landscape of biomedical research in neuroscience has changed dramatically in recent years as a result of spectacular progress in dynamic microscopy. However, the optical accessibility of deep brain structures or deeper regions of the surgically exposed hippocampus (a few 100 microns typically) remains limited, due to volumic aberrations created by the sample inhomogeneities. Adaptive optics can correct for these aberrations. Our goal is to realize a novel adaptive optics module dedicated to in vivo two-photon calcium imaging of the hippocampus. The key issue in adaptive optics is the ability to perform an accurate and reliable wavefront sensing. In two- photon microscopy indirect methods are required. Two families of approaches have been proposed so far, the modal sensorless technique and a method based on pupil segmentation. We present here a formal comparison of these approaches, in particular as a function of the amount of aberrations.

Citation Download Citation

Serge Meimon, Jean-Marc Conan, Laurent M. Mugnier, Vincent Michau, Rosa Cossart, and Arnaud Malvache "Adaptive optics for in vivo two-photon calcium imaging of neuronal networks", Proc. SPIE 8978, MEMS Adaptive Optics VIII, 89780C (7 March 2014); https://doi.org/10.1117/12.2044052

ACCESS THE FULL ARTICLE

INSTITUTIONAL
Select your institution to access the SPIE Digital Library.

SELECT YOUR INSTITUTION

PERSONAL
Sign in with your SPIE account to access your personal subscriptions or to use specific features such as save to my library, sign up for alerts, save searches, etc.

PERSONAL SIGN IN

No SPIE Account? Create one

PURCHASE THIS CONTENT

SUBSCRIBE TO DIGITAL LIBRARY

50 downloads per 1-year subscription

Members: $195

Non-members: $335 ADD TO CART

25 downloads per 1 - year subscription

Members: $145

Non-members: $250 ADD TO CART

PURCHASE SINGLE ARTICLE

Includes PDF, HTML & Video, when available

Members: $17.00

Non-members: $21.00 ADD TO CART

PROCEEDINGS
8 PAGES

DOWNLOAD PAPER SAVE TO MY LIBRARY

GET CITATION

RIGHTS & PERMISSIONS

Get copyright permission Get copyright permission on Copyright Marketplace

KEYWORDS

Sensors

Adaptive optics

Image segmentation

Wavefront sensors

In vivo imaging

Microscopy

Calcium

Show All Keywords

Keywords/Phrases

Search In:

Publication Years