The synchronous fluorescence spectra character of hypocrellin B with type I collagen under irradiation

Jucheng Zhang; Heping Yan; Mengting Yang; Yongzhen Feng; Yun Huang; Lingmei Li; Wei Liu

doi:10.1117/12.2068299

17 September 2014 The synchronous fluorescence spectra character of hypocrellin B with type I collagen under irradiation

Jucheng Zhang, Heping Yan, Mengting Yang, Yongzhen Feng, Yun Huang, Lingmei Li, Wei Liu

Author Affiliations +

Proceedings Volume 9230, Twelfth International Conference on Photonics and Imaging in Biology and Medicine (PIBM 2014); 923005 (2014) https://doi.org/10.1117/12.2068299
Event: Twelfth International Conference on Photonics and Imaging in Biology and Medicine (PIBM 2014), 2014, Wuhan, China

Abstract

The synchronous fluorescence (SF) technique can provide some useful information with the endogenous fluorophores in complex systems. The SF technique has been used in the characterization of the type I collagen in PBS solution (pH=7.4) and the photo-reaction of hypocrellin B (HB) with type I collagen in solution irradiation with the 475 nm light under saturated by oxygen, air and nitrogen respectively. The SF spectra show the peaks attribute to tyrosine residues (275 nm) and pyridinoline cross-link (325 nm) in collagen. The photo-induced reaction with HB causing the fluorescence quenching but no wavelength shift, this suggests the photo-reaction don’t changing the microenvironment of PYD cross-link. The fluorescence quenching rate is faster than others when the solution saturated by oxygen. The results imply that the photo-induced reaction is oxygen dependence.

Citation Download Citation

Jucheng Zhang, Heping Yan, Mengting Yang, Yongzhen Feng, Yun Huang, Lingmei Li, and Wei Liu "The synchronous fluorescence spectra character of hypocrellin B with type I collagen under irradiation", Proc. SPIE 9230, Twelfth International Conference on Photonics and Imaging in Biology and Medicine (PIBM 2014), 923005 (17 September 2014); https://doi.org/10.1117/12.2068299

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