Screening mTOR siRNA based on bioinformatics and detecting the transcription by the gold nanoparticle beacon

Caiping Tian; Yi Ma; Siwen Li; Yueqing Gu

doi:10.1117/12.2068706

17 September 2014 Screening mTOR siRNA based on bioinformatics and detecting the transcription by the gold nanoparticle beacon

Caiping Tian, Yi Ma, Siwen Li, Yueqing Gu

Author Affiliations +

Proceedings Volume 9230, Twelfth International Conference on Photonics and Imaging in Biology and Medicine (PIBM 2014); 92300D (2014) https://doi.org/10.1117/12.2068706
Event: Twelfth International Conference on Photonics and Imaging in Biology and Medicine (PIBM 2014), 2014, Wuhan, China

Abstract

Mammalian target of rapamycin (mTOR) as a key protein in PI3K-AKT-mTOR signaling pathway ,plays an important role in the tumor growth. The small interfering RNA (siRNA) of mTOR would decrease the expression of mTOR protein. In this study, we screened the mTOR siRNA sequence using MATLAB software and ascertained it based on BLAST. Then we imported it with the aid of Lipofectamine2000 into MCF-7 cancer cells where mTOR is over expression .And then we used a special hairpin deoxyribonucleic acid (DNA) for combining with the human mTOR mRNA to functionalize gold nanoparticles, which served as a molecule beacon for detecting human mTOR mRNA transcription. Laser scanning confocal microscope and Flow Cytometry data showed that the quenching efficiency was up to 90%,which are consistent with the RT-PCR measurement and Western. Compared to the previous approaches, this beacon has advantages of higher target to background ratio of detection. The strategy reported in this study is a promising approach for the intracellular measurement of the result of siRNA or protein expression in living cells, and has great potential in the study of drug screening and discovery.

Citation Download Citation

Caiping Tian, Yi Ma, Siwen Li, and Yueqing Gu "Screening mTOR siRNA based on bioinformatics and detecting the transcription by the gold nanoparticle beacon", Proc. SPIE 9230, Twelfth International Conference on Photonics and Imaging in Biology and Medicine (PIBM 2014), 92300D (17 September 2014); https://doi.org/10.1117/12.2068706

ACCESS THE FULL ARTICLE

INSTITUTIONAL
Select your institution to access the SPIE Digital Library.

SELECT YOUR INSTITUTION

PERSONAL
Sign in with your SPIE account to access your personal subscriptions or to use specific features such as save to my library, sign up for alerts, save searches, etc.

PERSONAL SIGN IN

No SPIE Account? Create one

PURCHASE THIS CONTENT

SUBSCRIBE TO DIGITAL LIBRARY

50 downloads per 1-year subscription

Members: $195

Non-members: $335 ADD TO CART

25 downloads per 1 - year subscription

Members: $145

Non-members: $250 ADD TO CART

PURCHASE SINGLE ARTICLE

Includes PDF, HTML & Video, when available

Members: $17.00

Non-members: $21.00 ADD TO CART

PROCEEDINGS
9 PAGES

DOWNLOAD PAPER SAVE TO MY LIBRARY

GET CITATION

RIGHTS & PERMISSIONS

Get copyright permission Get copyright permission on Copyright Marketplace

KEYWORDS

Gold

Nanoparticles

Flow cytometry

Luminescence

Proteins

Absorption

Cancer

Show All Keywords

Keywords/Phrases

Search In:

Publication Years