Monitoring wound healing by multiphoton tomography/endoscopy

Karsten König; Martin Weinigel; Rainer Bückle; Martin Kaatz; Christina Hipler; Katharina Zens; Stefan W. Schneider; Volker Huck

doi:10.1117/12.2078882

26 February 2015 Monitoring wound healing by multiphoton tomography/endoscopy

Karsten König, Martin Weinigel, Rainer Bückle, Martin Kaatz, Christina Hipler, Katharina Zens, Stefan W. Schneider, Volker Huck

Author Affiliations +

Proceedings Volume 9303, Photonic Therapeutics and Diagnostics XI; 93030F (2015) https://doi.org/10.1117/12.2078882
Event: SPIE BiOS, 2015, San Francisco, California, United States

Abstract

Certified clinical multiphoton tomographs are employed to perform rapid label-free high-resolution in vivo histology. Novel tomographs include a flexible 360° scan head attached to a mechano-optical arm for autofluorescence and SHG imaging as well as rigid two-photon GRIN microendoscope. Mitochondrial fluorescent NAD(P)H, fluorescent elastin, keratin, and melanin as well as SHG-active collagen can be imaged with submicron resolution in human skin. The system was employed to study the healing of chronic wounds (venous leg ulcer) and acute wounds (curettage of actinic or seborrheic keratosis) on a subcellular level. Furthermore, a flexible sterile foil as interface between wound and focusing optic was tested.

Citation Download Citation

Karsten König, Martin Weinigel, Rainer Bückle, Martin Kaatz, Christina Hipler, Katharina Zens, Stefan W. Schneider, and Volker Huck "Monitoring wound healing by multiphoton tomography/endoscopy", Proc. SPIE 9303, Photonic Therapeutics and Diagnostics XI, 93030F (26 February 2015); https://doi.org/10.1117/12.2078882

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