Tissue oxygen monitoring by photoacoustic lifetime imaging (PALI) and its application to image-guided photodynamic therapy (PDT)

Qi Shao; Ekaterina Morgounova; Shai Ashkenazi

doi:10.1117/12.2080609

11 March 2015 Tissue oxygen monitoring by photoacoustic lifetime imaging (PALI) and its application to image-guided photodynamic therapy (PDT)

Qi Shao, Ekaterina Morgounova, Shai Ashkenazi

Author Affiliations +

Proceedings Volume 9323, Photons Plus Ultrasound: Imaging and Sensing 2015; 932320 (2015) https://doi.org/10.1117/12.2080609
Event: SPIE BiOS, 2015, San Francisco, California, United States

Abstract

The oxygen partial pressure (pO2), which results from the balance between oxygen delivery and its consumption, is a key component of the physiological state of a tissue. Images of oxygen distribution can provide essential information for identifying hypoxic tissue and optimizing cancer treatment. Previously, we have reported a noninvasive in vivo imaging modality based on photoacoustic lifetime. The technique maps the excited triplet state of oxygen-sensitive dye, thus reflects the spatial and temporal distribution of tissue oxygen. We have applied PALI on tumor on small animals to identify hypoxia area. We also showed that PALI is able monitor changes of tissue oxygen, in an acute ischemia and breathing modulation model. Here we present our work on developing a treatment/imaging modality (PDT-PALI) that integrates PDT and a combined ultrasound/photoacoustic imaging system. The system provides real-time feedback of three essential parameters namely: tissue oxygen, light penetration in tumor location, and distribution of photosensitizer. Tissue oxygen imaging is performed by applying PALI, which relies on photoacoustic probing of oxygen-dependent, excitation lifetime of Methylene Blue (MB) photosensitizer. Lifetime information can also be used to generate image showing the distribution of photosensitizer. The level and penetration depth of PDT illumination can be deduced from photoacoustic imaging at the same wavelength. All images will be combined with ultrasound B-mode images for anatomical reference.

Citation Download Citation

Qi Shao, Ekaterina Morgounova, and Shai Ashkenazi "Tissue oxygen monitoring by photoacoustic lifetime imaging (PALI) and its application to image-guided photodynamic therapy (PDT)", Proc. SPIE 9323, Photons Plus Ultrasound: Imaging and Sensing 2015, 932320 (11 March 2015); https://doi.org/10.1117/12.2080609

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