Imaging of oxygenation in 3D tissue models with multi-modal phosphorescent probes

Dmitri B. Papkovsky; Ruslan I. Dmitriev; Sergei Borisov

doi:10.1117/12.2079269

5 March 2015 Imaging of oxygenation in 3D tissue models with multi-modal phosphorescent probes

Dmitri B. Papkovsky, Ruslan I. Dmitriev, Sergei Borisov

Proceedings Volume 9329, Multiphoton Microscopy in the Biomedical Sciences XV; 93290B (2015) https://doi.org/10.1117/12.2079269
Event: SPIE BiOS, 2015, San Francisco, California, United States

Abstract

Cell-penetrating phosphorescence based probes allow real-time, high-resolution imaging of O₂ concentration in respiring cells and 3D tissue models. We have developed a panel of such probes, small molecule and nanoparticle structures, which have different spectral characteristics, cell penetrating and tissue staining behavior. The probes are compatible with conventional live cell imaging platforms and can be used in different detection modalities, including ratiometric intensity and PLIM (Phosphorescence Lifetime IMaging) under one- or two-photon excitation. Analytical performance of these probes and utility of the O₂ imaging method have been demonstrated with different types of samples: 2D cell cultures, multi-cellular spheroids from cancer cell lines and primary neurons, excised slices from mouse brain, colon and bladder tissue, and live animals. They are particularly useful for hypoxia research, ex-vivo studies of tissue physiology, cell metabolism, cancer, inflammation, and multiplexing with many conventional fluorophors and markers of cellular function.

Citation Download Citation

Dmitri B. Papkovsky, Ruslan I. Dmitriev, and Sergei Borisov "Imaging of oxygenation in 3D tissue models with multi-modal phosphorescent probes", Proc. SPIE 9329, Multiphoton Microscopy in the Biomedical Sciences XV, 93290B (5 March 2015); https://doi.org/10.1117/12.2079269

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KEYWORDS

Tissues

3D modeling

Oxygen

3D image processing

Imaging systems

Live cell imaging

Brain

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