Standing-wave excitation of fluorescence in a laser-scanning microscope allows precise contour mapping of the red blood cell membrane

Rumelo Amor; Sumeet Mahajan; William  B. Amos; Gail McConnell

doi:10.1117/12.2075644

9 March 2015 Standing-wave excitation of fluorescence in a laser-scanning microscope allows precise contour mapping of the red blood cell membrane

Rumelo Amor, Sumeet Mahajan, William B. Amos, Gail McConnell

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Proceedings Volume 9330, Three-Dimensional and Multidimensional Microscopy: Image Acquisition and Processing XXII; 933010 (2015) https://doi.org/10.1117/12.2075644
Event: SPIE BiOS, 2015, San Francisco, California, United States

Abstract

We demonstrate fluorescence excitation at multiple planes in a laser-scanning microscope by using the standing wave from a mirror placed close to the specimen. We have observed precise modulation of the standing waves close to a mirror, with a frequency proportional to the Stokes shift, corresponding to a moiré pattern between the excitation and emission standing-wave fields. We use standing-wave excitation to plot the exact contour maps of the red blood cell membrane, with an axial resolution of ≈90 nm. The method may prove useful in the study of diseases which involve the surface membrane of red blood cells.

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Rumelo Amor, Sumeet Mahajan, William B. Amos, and Gail McConnell "Standing-wave excitation of fluorescence in a laser-scanning microscope allows precise contour mapping of the red blood cell membrane", Proc. SPIE 9330, Three-Dimensional and Multidimensional Microscopy: Image Acquisition and Processing XXII, 933010 (9 March 2015); https://doi.org/10.1117/12.2075644

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KEYWORDS

Blood

Mirrors

Microscopes

Moire patterns

Electroluminescent displays

Modulation

Glasses

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