Determination of the persistence length of actin filaments on microcontact printed myosin patterns

Joanna Hajne; Kristi L. Hanson; Harm van Zalinge; Dan V. Nicolau Jr.; Dan V. Nicolau

doi:10.1117/12.2175772

11 March 2015 Determination of the persistence length of actin filaments on microcontact printed myosin patterns

Joanna Hajne, Kristi L. Hanson, Harm van Zalinge, Dan V. Nicolau Jr., Dan V. Nicolau

Proceedings Volume 9337, Nanoscale Imaging, Sensing, and Actuation for Biomedical Applications XII; 93370G (2015) https://doi.org/10.1117/12.2175772
Event: SPIE BiOS, 2015, San Francisco, California, United States

Abstract

Protein molecular motors, which convert chemical energy into kinetic energy, are prime candidates for use in nanodevice in which active transport is required. To be able to design these devices it is essential that the properties of the cytoskeletal filaments propelled by the molecular motors are well established. Here we used micro-contact printed BSA to limit the amount of HMM that can adsorb creating a tightly confined pathway for the filaments to travel. Both the image and statistical analysis of the movement of the filaments through these structures have been used to new insights into the motility behaviour of actomyosin on topographically homogenous, but motor-heterogeneous planar systems. It will be shown that it is possible to determine the persistence length of the filaments and that it is related to the amount of locally adsorbed HMM. This provides a basis that can be used to optimize the design of future nanodevices incorporating the actomyosin system for the active transport.

Citation Download Citation

Joanna Hajne, Kristi L. Hanson, Harm van Zalinge, Dan V. Nicolau Jr., and Dan V. Nicolau "Determination of the persistence length of actin filaments on microcontact printed myosin patterns", Proc. SPIE 9337, Nanoscale Imaging, Sensing, and Actuation for Biomedical Applications XII, 93370G (11 March 2015); https://doi.org/10.1117/12.2175772

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KEYWORDS

Nanostructures

Proteins

Head

Glasses

Image analysis

Luminescence

Statistical analysis

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