Label-free oligonucleotide biosensor based on dual-peak long period fiber grating

Xianfeng Chen; Chen Liu; Marcos R. Cardoso; Cleber R. Mendonça; David A. Nagel; Anna V. Hine; Lin Zhang

doi:10.1117/12.2207869

7 March 2016 Label-free oligonucleotide biosensor based on dual-peak long period fiber grating

Xianfeng Chen, Chen Liu, Marcos R. Cardoso, Cleber R. Mendonça, David A. Nagel, Anna V. Hine, Lin Zhang

Proceedings Volume 9702, Optical Fibers and Sensors for Medical Diagnostics and Treatment Applications XVI; 97020S (2016) https://doi.org/10.1117/12.2207869
Event: SPIE BiOS, 2016, San Francisco, California, United States

Abstract

We report the simplification and development of biofunctionalization methodology based on one-step 1-ethyl-3-(3- dimethylaminopropyl)carbodiimide (EDC)-mediated reaction. The dual-peak long period grating (dLPG) has been demonstrated its inherent ultrahigh sensitivity to refractive index (RI), achieving 50-fold improvement in RI sensitivity over a standard LPG sensor used in low RI range. With the simple and efficient immobilization of unmodified oligonucleotides on sensor surface, dLPG-based biosensor has been used to monitor the hybridization of complementary oligonucleotides showing a detectable oligonucleotide concentration of 4 nM with the advantages of label-free, real-time, and ultrahigh sensitivity.

Citation Download Citation

Xianfeng Chen, Chen Liu, Marcos R. Cardoso, Cleber R. Mendonça, David A. Nagel, Anna V. Hine, and Lin Zhang "Label-free oligonucleotide biosensor based on dual-peak long period fiber grating", Proc. SPIE 9702, Optical Fibers and Sensors for Medical Diagnostics and Treatment Applications XVI, 97020S (7 March 2016); https://doi.org/10.1117/12.2207869

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