Fluorescent detection of C-reactive protein using polyamide beads

Shreesha Jagadeesh; Lu Chen; Stewart Aitchison

doi:10.1117/12.2211373

4 March 2016 Fluorescent detection of C-reactive protein using polyamide beads

Shreesha Jagadeesh, Lu Chen, Stewart Aitchison

Proceedings Volume 9715, Optical Diagnostics and Sensing XVI: Toward Point-of-Care Diagnostics; 971505 (2016) https://doi.org/10.1117/12.2211373
Event: SPIE BiOS, 2016, San Francisco, California, United States

Abstract

Bacterial infection causes Sepsis which is one of the leading cause of mortality in hospitals. This infection can be quantified from blood plasma using C - reactive protein (CRP). A quick diagnosis at the patient’s location through Point-of- Care (POC) testing could give doctors the confidence to prescribe antibiotics. In this paper, the development and testing of a bead-based procedure for CRP quantification is described. The size of the beads enable them to be trapped in wells without the need for magnetic methods of immobilization. Large (1.5 mm diameter) Polyamide nylon beads were used as the substrate for capturing CRP from pure analyte samples. The beads captured CRP either directly through adsorption or indirectly by having specific capture antibodies on their surface. Both methods used fluorescent imaging techniques to quantify the protein. The amount of CRP needed to give a sufficient fluorescent signal through direct capture method was found suitable for identifying bacterial causes of infection. Similarly, viral infections could be quantified by the more sensitive indirect capture method. This bead-based assay can be potentially integrated as a disposable cartridge in a POC device due to its passive nature and the small quantities needed.

Conference Presentation

Citation Download Citation

Shreesha Jagadeesh, Lu Chen, and Stewart Aitchison "Fluorescent detection of C-reactive protein using polyamide beads", Proc. SPIE 9715, Optical Diagnostics and Sensing XVI: Toward Point-of-Care Diagnostics, 971505 (4 March 2016); https://doi.org/10.1117/12.2211373

ACCESS THE FULL ARTICLE

INSTITUTIONAL
Select your institution to access the SPIE Digital Library.

SELECT YOUR INSTITUTION

PERSONAL
Sign in with your SPIE account to access your personal subscriptions or to use specific features such as save to my library, sign up for alerts, save searches, etc.

PERSONAL SIGN IN

No SPIE Account? Create one

PURCHASE THIS CONTENT

SUBSCRIBE TO DIGITAL LIBRARY

50 downloads per 1-year subscription

Members: $195

Non-members: $335 ADD TO CART

25 downloads per 1 - year subscription

Members: $145

Non-members: $250 ADD TO CART

PURCHASE SINGLE ARTICLE

Includes PDF, HTML & Video, when available