Engineering molecularly-active nanoplasmonic surfaces for DNA detection via colorimetry and Raman scattering

Esmaeil Heydari; Samuel Mabbott; David Thompson; Duncan Graham; Jonathan M. Cooper; Alasdair W. Clark

doi:10.1117/12.2209108

22 April 2016 Engineering molecularly-active nanoplasmonic surfaces for DNA detection via colorimetry and Raman scattering

Esmaeil Heydari, Samuel Mabbott, David Thompson, Duncan Graham, Jonathan M. Cooper, Alasdair W. Clark

Author Affiliations +

Proceedings Volume 9721, Nanoscale Imaging, Sensing, and Actuation for Biomedical Applications XIII; 972105 (2016) https://doi.org/10.1117/12.2209108
Event: SPIE BiOS, 2016, San Francisco, California, United States

Abstract

We report a novel nanophotonic biosensor surface capable of both colorimetric detection and Raman-scattered detection of DNA infection markers at extreme sensitivities. Combining direct-write lithography, dip-pen nanolithography based DNA patterning, and molecular self-assembly, we create molecularly-active plasmonic nanostructures onto which metallic nanoparticles are located via DNA-hybridization. Arraying these structures enables optical surfaces that change state when contacted by specific DNA sequences; shifting the surface color while simultaneously generating strong Raman-scattering signals. Patterning the DNA markers onto the plasmonic surface as micro-scale symbols results in easily identifiable color shifts, making this technique applicable to multiplexed lab-on-a-chip and point-of-care diagnostic applications.

Conference Presentation

Citation Download Citation

Esmaeil Heydari, Samuel Mabbott, David Thompson, Duncan Graham, Jonathan M. Cooper, and Alasdair W. Clark "Engineering molecularly-active nanoplasmonic surfaces for DNA detection via colorimetry and Raman scattering", Proc. SPIE 9721, Nanoscale Imaging, Sensing, and Actuation for Biomedical Applications XIII, 972105 (22 April 2016); https://doi.org/10.1117/12.2209108

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