Development of cadmium-free quantum dot for intracellular labelling through electroporation or lipid-calcium-phosphate

Ying-Feng Liu; Wei-Ling Hung; Tzh-Yin Hou; Hsiu-Ying Huang; Cheng-An J. Lin

doi:10.1117/12.2225864

19 April 2016 Development of cadmium-free quantum dot for intracellular labelling through electroporation or lipid-calcium-phosphate

Ying-Feng Liu, Wei-Ling Hung, Tzh-Yin Hou, Hsiu-Ying Huang, Cheng-An J. Lin

Author Affiliations +

Proceedings Volume 9884, Nanophotonics VI; 988428 (2016) https://doi.org/10.1117/12.2225864
Event: SPIE Photonics Europe, 2016, Brussels, Belgium

Abstract

Traditional fluorescent labelling techniques has severe photo-bleaching problem such as organic dyes and fluorescent protein. Quantum dots made up of traditional semiconductor (CdSe/ZnS) material has sort of biological toxicity. This research has developed novel Cd-free quantum dots divided into semiconductor (Indium phosphide, InP) and noble metal (Gold). Former has lower toxicity compared to traditional quantum dots. Latter consisting of gold (III) chloride (AuCl3) and toluene utilizes sonochemical preparation and different stimulus to regulate fluorescent wavelength. Amphoteric macromolecule surface technology and ligand Exchange in self-Assembled are involved to develop hydrophilic nanomaterials which can regulate the number of grafts per molecule of surface functional groups. Calcium phosphate (CaP) nanoparticle (NP) with an asymmetric lipid bilayer coating technology developed for intracellular delivery and labelling has synthesized Cd-free quantum dots possessing high brightness and multi-fluorescence successfully. Then, polymer coating and ligand exchange transfer to water-soluble materials to produce liposome nanomaterials as fluorescent probes and enhancing medical applications of nanotechnology.

Citation Download Citation

Ying-Feng Liu, Wei-Ling Hung, Tzh-Yin Hou, Hsiu-Ying Huang, and Cheng-An J. Lin "Development of cadmium-free quantum dot for intracellular labelling through electroporation or lipid-calcium-phosphate", Proc. SPIE 9884, Nanophotonics VI, 988428 (19 April 2016); https://doi.org/10.1117/12.2225864

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