Application of lens-free quantitative large-area agglutination assay to inflammatory disease and SARS-CoV-2

Colin J. Potter; Zhen Xiong; Euan McLeod

doi:10.1117/12.2607780

7 March 2022 Application of lens-free quantitative large-area agglutination assay to inflammatory disease and SARS-CoV-2

Colin J. Potter, Zhen Xiong, Euan McLeod

Author Affiliations +

Proceedings Volume PC11940, Optical Methods for Tumor Treatment and Detection: Mechanisms and Techniques in Photodynamic and Photobiomodulation Therapy XXX; (2022) https://doi.org/10.1117/12.2607780
Event: SPIE BiOS, 2022, San Francisco, California, United States

Abstract

Biodetection of inflammatory and disease-related targets is achieved using a portable quantitative large-area binding (QLAB) sensor. This sensor utilizes lens-free holographic microscopy, computational image processing involving pixel super-resolution, a custom LED array and microfluidic chip, and automated feature quantification algorithm to detect microbead agglutination in the presence of a target molecule or pathogen in solution. Here, we discuss a recent application involving biodetection of interferon-gamma (IFN-gamma) in solution where the QLAB sensor achieved a sensitivity of < 3 ng/mL We also discuss current work using this technology to sense SARS-CoV-2 in a point-of-care setting.

Conference Presentation

Citation Download Citation

Colin J. Potter, Zhen Xiong, and Euan McLeod "Application of lens-free quantitative large-area agglutination assay to inflammatory disease and SARS-CoV-2", Proc. SPIE PC11940, Optical Methods for Tumor Treatment and Detection: Mechanisms and Techniques in Photodynamic and Photobiomodulation Therapy XXX, (7 March 2022); https://doi.org/10.1117/12.2607780

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