Real time oxygen mapping in vivo during pre-clinical radiotherapy using delayed fluorescence of protoporphyrin IX

Arthur Pétusseau; Mahbubur Rahman; Petr Bruza; Sergei Vinogradov; Brian Pogue

doi:10.1117/12.2608712

7 March 2022 Real time oxygen mapping in vivo during pre-clinical radiotherapy using delayed fluorescence of protoporphyrin IX

Arthur Pétusseau, Mahbubur Rahman, Petr Bruza, Sergei Vinogradov, Brian Pogue

Author Affiliations +

Proceedings Volume PC11940, Optical Methods for Tumor Treatment and Detection: Mechanisms and Techniques in Photodynamic and Photobiomodulation Therapy XXX; (2022) https://doi.org/10.1117/12.2608712
Event: SPIE BiOS, 2022, San Francisco, California, United States

Abstract

Delayed fluorescence (DF) from the endogenous molecule protoporphyrin IX (PpIX) has been shown to be a truly unique reporter of the local oxygen partial pressure in tissue, with the origin of the signal comes from within or near the mitochondria of the cells. Here we developed a wide field system capable of real-time imaging of oxygen. During photodynamic therapy and FLASH radiation therapy (RT), oxygen is rapidly consumed in the targeted tissues and oxygen depletion is commonly known to occur. In both cases, we have used the pO2 imaging system to map out localized changes during treatment. We showed that mapping of partial oxygen pressure was possible at video rates up to 10 fps. This work also revealed that intracellular oxygen depletion is consequent during FLASH, bringing further clarification on the technique’s mechanism.

Conference Presentation

Citation Download Citation

Arthur Pétusseau, Mahbubur Rahman, Petr Bruza, Sergei Vinogradov, and Brian Pogue "Real time oxygen mapping in vivo during pre-clinical radiotherapy using delayed fluorescence of protoporphyrin IX", Proc. SPIE PC11940, Optical Methods for Tumor Treatment and Detection: Mechanisms and Techniques in Photodynamic and Photobiomodulation Therapy XXX, (7 March 2022); https://doi.org/10.1117/12.2608712

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