PERSONAL Sign in with your SPIE account to access your personal subscriptions or to use specific features such as save to my library, sign up for alerts, save searches, etc.
F-PTIR is described theoretically and demonstrated experimentally for f high spatial resolution chemical microscopy based on mid-infrared absorption spectroscopy. In F-PTIR, heat produced locally by infrared absorption results in reductions in fluorescence through temperature-dependent changes in quantum efficiency. The point-spread function for F-PTIR is identical to that of fluorescence, simplifying the interpretation of image contrast relative to alternative scattering/refraction based photothermal detection methods. Fluorescence labeling enables infrared spectroscopy just in the regions associated with the label, providing dual selectivity for local chemical environments. F-PTIR spectroscopy was used to identify phase-separated domains within pharmaceutical materials impacting bioavailability and formulations design.
Garth J. Simpson
"Chemical imaging by fluorescence-detected photothermal mid-infrared (F-PTIR) microscopy", Proc. SPIE PC11957, Biomedical Vibrational Spectroscopy 2022: Advances in Research and Industry, PC119570I (7 March 2022); https://doi.org/10.1117/12.2610297
ACCESS THE FULL ARTICLE
INSTITUTIONAL Select your institution to access the SPIE Digital Library.
PERSONAL Sign in with your SPIE account to access your personal subscriptions or to use specific features such as save to my library, sign up for alerts, save searches, etc.
The alert did not successfully save. Please try again later.
Garth J. Simpson, "Chemical imaging by fluorescence-detected photothermal mid-infrared (F-PTIR) microscopy," Proc. SPIE PC11957, Biomedical Vibrational Spectroscopy 2022: Advances in Research and Industry, PC119570I (7 March 2022); https://doi.org/10.1117/12.2610297