PERSONAL Sign in with your SPIE account to access your personal subscriptions or to use specific features such as save to my library, sign up for alerts, save searches, etc.
Ultrasonic methods hold immense promise for functional measurements and interventions during in vivo studies due to the beneficial scattering properties of acoustic waves. Here we present the design and application of a system that integrates into standard multiphoton microscopes, which co-linearly combines two-photon fluorescence imaging and precise acoustic sonication. By applying this system across a range of samples, we observed a novel gigantic Acousto-Optic (AO) modulation of two-photon excited fluorescence, and also study the mechanisms of functional ultrasound neuromodulation, an emerging non-invasive neurostimulation technique. This work demonstrates simple photonic/acoustic methods to study novel phenomenon in the awake mouse.
Justin Little,Behnam Tayebi,Yi Yuan,Michal Balberg, andShy Shoham
"Collinear ultrasonic and multiphoton focusing for advanced functional imaging and stimulation (Conference Presentation)", Proc. SPIE PC12384, Multiphoton Microscopy in the Biomedical Sciences XXIII, PC123840R (15 March 2023); https://doi.org/10.1117/12.2650811
ACCESS THE FULL ARTICLE
INSTITUTIONAL Select your institution to access the SPIE Digital Library.
PERSONAL Sign in with your SPIE account to access your personal subscriptions or to use specific features such as save to my library, sign up for alerts, save searches, etc.
The alert did not successfully save. Please try again later.
Justin Little, Behnam Tayebi, Yi Yuan, Michal Balberg, Shy Shoham, "Collinear ultrasonic and multiphoton focusing for advanced functional imaging and stimulation (Conference Presentation)," Proc. SPIE PC12384, Multiphoton Microscopy in the Biomedical Sciences XXIII, PC123840R (15 March 2023); https://doi.org/10.1117/12.2650811