Single objective light sheet microscopy with multi-direction excitation and structured light (Conference Presentation)

Yang Liu; Ming Song; Bingxi Liu; Peter A. Kner

doi:10.1117/12.2649229

15 March 2023 Single objective light sheet microscopy with multi-direction excitation and structured light (Conference Presentation)

Yang Liu, Ming Song, Bingxi Liu, Peter A. Kner

Author Affiliations +

Proceedings Volume PC12385, Three-Dimensional and Multidimensional Microscopy: Image Acquisition and Processing XXX; PC1238505 (2023) https://doi.org/10.1117/12.2649229
Event: SPIE BiOS, 2023, San Francisco, California, United States

Abstract

Light-Sheet Fluorescence Microscopy (LSFM) has proven to be an excellent tool for imaging the nervous system zebrafish and other model organisms. However, these LSFMs typically have low resolution and suffer from striping artifacts. Here, we present a light sheet system using a novel single-objective light-sheet geometry to allow for multi-direction light-sheet illumination with structured light. This geometry is designed to mitigate striping artifacts and achieve lateral resolutions of better than 200 nm over a 277 × 277 × 100 µm3 volume. We demonstrate the system by imaging the central nervous system of dissected Drosophila embryos as well as cerebellum organoids.

Conference Presentation

Citation Download Citation

Yang Liu, Ming Song, Bingxi Liu, and Peter A. Kner "Single objective light sheet microscopy with multi-direction excitation and structured light (Conference Presentation)", Proc. SPIE PC12385, Three-Dimensional and Multidimensional Microscopy: Image Acquisition and Processing XXX, PC1238505 (15 March 2023); https://doi.org/10.1117/12.2649229

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