Design and fabrication of a GRIN-axicon for Bessel-Gauss beam generation and applications to fast volumetric imaging (Conference Presentation)

Mireille Quémener; Pierre Girard-Collins; Alexandra Alain-Beaudoin; Nicolas Grégoire; Steeve Morency; Michèle Desjardins; Daniel C. Côté; Simon Thibault

doi:10.1117/12.2648999

15 March 2023 Design and fabrication of a GRIN-axicon for Bessel-Gauss beam generation and applications to fast volumetric imaging (Conference Presentation)

Mireille Quémener, Pierre Girard-Collins, Alexandra Alain-Beaudoin, Nicolas Grégoire, Steeve Morency, Michèle Desjardins, Daniel C. Côté, Simon Thibault

Author Affiliations +

Proceedings Volume PC12385, Three-Dimensional and Multidimensional Microscopy: Image Acquisition and Processing XXX; PC123850K (2023) https://doi.org/10.1117/12.2648999
Event: SPIE BiOS, 2023, San Francisco, California, United States

Abstract

We introduce the GRIN-axicon, a new low-cost optical component that is easy to manufacture and could replace the axicon in various setups such as a two-photon microscope. In neuroscience, the imaging of in vivo samples requires high temporal resolution in order to capture the interactions between neurons located at different depths in the tissue. To achieve this, the use of an axicon lens increases the depth of field of the microscope and reduces the number of scans to be performed. However, the axicon is difficult to manufacture and generally has defects on the tip of the cone, thus degrading the quality of the resultant Bessel-Gauss beam.

Conference Presentation

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Mireille Quémener, Pierre Girard-Collins, Alexandra Alain-Beaudoin, Nicolas Grégoire, Steeve Morency, Michèle Desjardins, Daniel C. Côté, and Simon Thibault "Design and fabrication of a GRIN-axicon for Bessel-Gauss beam generation and applications to fast volumetric imaging (Conference Presentation)", Proc. SPIE PC12385, Three-Dimensional and Multidimensional Microscopy: Image Acquisition and Processing XXX, PC123850K (15 March 2023); https://doi.org/10.1117/12.2648999

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KEYWORDS

Axicons

Microscopes

Optics manufacturing

Manufacturing

Microscopy

Neurons

Neuroscience

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