Sample-conjugate sensorless scatter compensation in two-photon fluorescence microscopy (Conference Presentation)

Alexander Jesacher; Maximilian Sohmen; Juan Munoz-Bolanos; Monika Ritsch-Marte

doi:10.1117/12.2654654

16 March 2023 Sample-conjugate sensorless scatter compensation in two-photon fluorescence microscopy (Conference Presentation)

Alexander Jesacher, Maximilian Sohmen, Juan Munoz-Bolanos, Monika Ritsch-Marte

Author Affiliations +

Proceedings Volume PC12388, Adaptive Optics and Wavefront Control for Biological Systems IX; PC1238801 (2023) https://doi.org/10.1117/12.2654654
Event: SPIE BiOS, 2023, San Francisco, California, United States

Abstract

Recently, our group has introduced Dynamic Adaptive Scattering Compensation Holography (DASH), a sensorless wavefront correction method for nonlinear imaging, which allows retrieving even strong wavefront aberrations in short times.The possibility to compensate the adverse effects of high spatial frequency volumetric scatterers shifts yet another problem into the focus: the small size of corrected sample regions, which sometimes measure only a few µm across. Real-world biological applications, however, demand the imaging of larger fields, making it highly important to find ways of enlarging these correction zones. We present a sample-conjugate version of DASH, which addresses this problem in two ways: While sample-conjugation is naturally better suited to compensate the effect of certain scatterers, the use of a large pixel count SLM further allows applying multiple correction patterns simultaneously.

Conference Presentation

Citation Download Citation

Alexander Jesacher, Maximilian Sohmen, Juan Munoz-Bolanos, and Monika Ritsch-Marte "Sample-conjugate sensorless scatter compensation in two-photon fluorescence microscopy (Conference Presentation)", Proc. SPIE PC12388, Adaptive Optics and Wavefront Control for Biological Systems IX, PC1238801 (16 March 2023); https://doi.org/10.1117/12.2654654

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KEYWORDS

Sensors

Two photon excitation microscopy

Holography

Scattering compensation

Sensor calibration

Spatial frequencies

Spatial light modulators

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