3D image acquisition with multispectral FLIM and compressed sensing (Conference Presentation)

Anneliese L. Jarman; Hanning Mai; Conor Treacy; Ahmet Erdogan; Neil Finlayson; Claire Wells; Robert Henderson; Simon Poland

doi:10.1117/12.2649690

16 March 2023 3D image acquisition with multispectral FLIM and compressed sensing (Conference Presentation)

Anneliese L. Jarman, Hanning Mai, Conor Treacy, Ahmet Erdogan, Neil Finlayson, Claire Wells, Robert Henderson, Simon Poland

Author Affiliations +

Proceedings Volume PC12390, High-Speed Biomedical Imaging and Spectroscopy VIII; PC123900R (2023) https://doi.org/10.1117/12.2649690
Event: SPIE BiOS, 2023, San Francisco, California, United States

Abstract

Precisely characterising and quantifying interactions between tumour cells and their environment to understand metastatic mechanisms requires a multi-dimensional, high-speed imaging system. To this end, we report on the development of a compressive full spectrum fluorescence lifetime microscope that exploits a novel SPAD line sensor and a DMD to enable monitoring of dynamic sub-cellular interactions. At no cost to its temporal performance, the hyperspectral nature of the system helps to improve unmixing and, crucially, can detect the small spectral changes in the emission of fluorescent probes and intrinsic fluorophores that can occur in complex environments.

Conference Presentation

Citation Download Citation

Anneliese L. Jarman, Hanning Mai, Conor Treacy, Ahmet Erdogan, Neil Finlayson, Claire Wells, Robert Henderson, and Simon Poland "3D image acquisition with multispectral FLIM and compressed sensing (Conference Presentation)", Proc. SPIE PC12390, High-Speed Biomedical Imaging and Spectroscopy VIII, PC123900R (16 March 2023); https://doi.org/10.1117/12.2649690

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