Fast-speed tilted-plane volumetric imaging of freshly excised human skin tissues

Zhenguo Wu; Tashmeeta Ahad; Sunil Kalia; Harvey Lui; Shujian Li; Jianhua Zhao; Haishan Zeng

doi:10.1117/12.3008997

13 March 2024 Fast-speed tilted-plane volumetric imaging of freshly excised human skin tissues

Zhenguo Wu, Tashmeeta Ahad, Sunil Kalia, Harvey Lui, Shujian Li, Jianhua Zhao, Haishan Zeng

Author Affiliations +

Proceedings Volume PC12816, Photonics in Dermatology and Plastic Surgery 2024; PC128160L (2024) https://doi.org/10.1117/12.3008997
Event: SPIE BiOS, 2024, San Francisco, California, United States

Abstract

Fast-speed volumetric imaging methods are desired for both multiphoton and confocal microscopy. A tilted-plane volumetric imaging and reconstruction method was developed to achieve fast volumetric imaging of freshly biopsied/excised human skin tissue based on only xy laser scanning. A volume that covers a surface area of 10 mm × 8 mm and a thickness of 80 um can be acquired in 9 minutes. The large surface area was achieved by stitching multiple volume strips and each volume strip was reconstructed from a sequence of frames acquired along the tissue surface at a frame rate of 198 frames per second and each frame was captured in an imaging plane that is tilted relative to the tissue surface. The imaging method was implemented on a multimodality imaging system to generate confocal reflectance, second harmonic, and two-photon fluorescence volumes simultaneously and give complementary information of the excised tissue. Application in skin cancer surgery guidance will be pursued in the future.

Conference Presentation

Citation Download Citation

Zhenguo Wu, Tashmeeta Ahad, Sunil Kalia, Harvey Lui, Shujian Li, Jianhua Zhao, and Haishan Zeng "Fast-speed tilted-plane volumetric imaging of freshly excised human skin tissues", Proc. SPIE PC12816, Photonics in Dermatology and Plastic Surgery 2024, PC128160L (13 March 2024); https://doi.org/10.1117/12.3008997

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